|
January 2006
 Job title and general description
Technician Level III UBC Caenorhabditis elegans Genomics Facility
- Expression Group
To study the patterns
of expression of specific genes at various stages in C. elegans
development, we incorporate GFP promoter fusions. This creates
a green fluorescence in the specific tissues of the worm where
the gene is being expressed. In this way we are able to visualize
the genes expression pattern.
I observe the worms from the embryo stage right through
to adulthood using a variety of microscopes. I use a dissecting
microscope to observe large populations of worms. I can then
pick out individual worms to investigate in detail, and take
images of them, on a compound microscope. To create a 3-D
projection of the subject I use a confocal microscope to take
images of the worm across multiple planes and the computer
software generates the projection. By observing where and
when the green fluorescence is visible I gather information
on the location and timing of expression of specific genes.
What
specific skills and qualities are needed to do your job?
My job requires a keen desire to learn and the ability to
work independently.
What
do you like most about your work?
My boss is great. He gives me the freedom to explore and he
is very encouraging. I enjoy working on my own project and
not having to depend on other people.
What don’t you like about your job?
The part that I like least about my job is that is does get
quite repetitive.
Where
do you see your career going from here?
I plan to go on to Grad school and continue to work in this
lab.
What
is the range in salary that comes with your job?
This type of job pays $35,000- $40,000.
When
did you know you wanted to work in sciences?
I have been interested in science, especially genetics, since
I was very young.
What
is your educational background?
So far I have a B.Sc. from UBC in Biology with a specialty
in Genetics.
What
was your favorite course(s) and why?
My favorite course was Microbiology 409, Microbial Genetics.
We worked on experimental design and did some work with plasmids.
I really enjoyed this.
What
other hobbies and interests do you have?
I curl on a team. I also enjoy camping and other outdoor activities.
Do you have any
advice for young people thinking about getting into your line
of work?
The field of science is competitive. It is very useful to
make good contacts with people like university professors
and other people in the field. Volunteer work is also very
important. Good referrals are essential in helping you move
ahead in this field.
|
